He is travelling from his home in Dundee to Bute House in Edinburgh, the first minister’s official residence, for a press conference at 12:00.
The SNP leader has been under pressure since he ended a power-sharing deal with the Scottish Greens last week.
Opposition parties in the Scottish Parliament had tabled two confidence votes – one in the first minister and another in the SNP government.
Mr Yousaf has been struggling to secure support to lead a minority government.
He had written to Scotland’s opposition parties asking them to find “common ground” ahead of the confidence votes.
The first minister’s decision to end the Bute House Agreement – the power-sharing deal with the Scottish Greens – followed a backlash over the SNP’s decision to scrap 2030 climate targets.
The Greens accused Mr Yousaf of “selling out future generations” and had themselves been due vote on whether to remain in power with the SNP.
The SNP has 63 MSPs in the 129 seat parliament. If the seven Green MSPs vote against him, he is reliant on support from sole Alba party MSP, Ash Regan, to continue in his role.
Mr Yousaf left his home in Dundee to travel to Bute House
Mr Yousaf, the MSP for Glasgow Pollok, has reportedly ruled out cutting a deal with Alba, a pro-independence party formed by former First Minister Alex Salmond after he broke from the SNP.
Alba’s support would lead to a 64:64 tied vote in which case the presiding officer would be expected to vote to maintain the status quo.
The motion of no confidence in him personally is not binding, but if he lost he would come under intense pressure to step down.
If he lost the government vote, MSPs would have 28 days to vote for a new first minister or automatically trigger a Scottish Parliament election.
Scottish Labour has said the motion of no-confidence in the Scottish government would remain tabled even if Mr Yousaf resigns.
The Scottish Greens had been hoping to become the decision-makers on the future of the Bute House Agreement.
Jen Bell, co-convenor of the party’s LGBT arm Rainbow Greens, called for a vote on the power-sharing agreement, which was scheduled to take place at the end of May.
Some party members were unhappy about the Scottish government considering implementing the recommendations a landmark review of child gender services in England by paediatrician Dr Hilary Cass.
Green party co-leader Patrick Harvie told BBC Radio’s Good Morning Scotland programme there was “distress” among members that children would not be able to get medical treatment to change gender after NHS Scotland paused puberty blockers for under-18s.